phospho-BCAR1 (Tyr410) Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6224R-FITC |
| Product Name | phospho-BCAR1 (Tyr410) Polyclonal Antibody, FITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | Will recognize BCAR1(Tyr414) in mouse and BCAR1(Tyr508) in rat |
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Horse |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human BCAR1 around the phosphorylation site of Tyr410. |
| Modification Site | Tyr410 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9564 |
| Swiss Prot | P56945 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | CAS; CAS1; CASS1; CRKAS; P130Cas; Breast cancer anti-estrogen resistance protein 1; CRK-associated substrate; Cas scaffolding protein family member 1; BCAR1 |
| Background | Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-BCAR1 (Tyr410) Polyclonal Antibody, FITC Conjugated (bs-6224R-FITC) validated for, and at what dilution?
bs-6224R-FITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-BCAR1 (Tyr410) Polyclonal Antibody, FITC Conjugated (bs-6224R-FITC) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Horse.
How should bs-6224R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-BCAR1 (Tyr410) Polyclonal Antibody, FITC Conjugated (bs-6224R-FITC)?
KLH conjugated synthetic phosphopeptide derived from human BCAR1 around the phosphorylation site of Tyr410.
What are the excitation and emission wavelengths of the FITC conjugate (bs-6224R-FITC)?
FITC on bs-6224R-FITC: excitation / emission 494nm/518nm.