MAGEF1 Polyclonal Antibody, Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-6231R-Cy5 |
| Product Name | MAGEF1 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Dog, Pig |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MAGEF1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 64110 |
| Subcellular location | Secreted, Extracellular matrix |
| Synonyms | MAGE F1 antigen; MAGEF 1; Melanoma antigen family F, 1; Melanoma associated antigen F1; MGC19617; MAGF1_HUMAN. |
| Background | MAGEF1 is a member of the MAGE (melanoma antigen gene) superfamily.Most known members of the MAGE superfamily are expressed in tumors, testis and fetal tissues, which has been described as a cancer/testis or "CT" expression pattern. MAGEF1, however, is expressed in all adult and fetal tissues tested, as well as in many tumor types including ovarian, breast, cervical, melanoma and leukemia. The coding region of MAGE-F1 is contained within a single exon and includes a microsatellite repeat. Several MAGE genes are ubiquitously expressed suggesting a role for MAGE encoded proteins in normal cell physiology. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is MAGEF1 Polyclonal Antibody, Cy5 Conjugated (bs-6231R-Cy5) validated for, and at what dilution?
bs-6231R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-6231R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MAGEF1 Polyclonal Antibody, Cy5 Conjugated (bs-6231R-Cy5)?
KLH conjugated synthetic peptide derived from human MAGEF1
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-6231R-Cy5)?
Cy5 on bs-6231R-Cy5: excitation / emission 625,650nm/670nm.