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MAGEF1 Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Dog
  • Pig
Overview
Catalog # bs-6231R-PE-100uL
Product Name MAGEF1 Polyclonal Antibody, PE Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Dog, Pig
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human MAGEF1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 64110
Subcellular location Secreted, Extracellular matrix
Synonyms MAGE F1 antigen; MAGEF 1; Melanoma antigen family F, 1; Melanoma associated antigen F1; MGC19617; MAGF1_HUMAN.
Background MAGEF1 is a member of the MAGE (melanoma antigen gene) superfamily.Most known members of the MAGE superfamily are expressed in tumors, testis and fetal tissues, which has been described as a cancer/testis or "CT" expression pattern. MAGEF1, however, is expressed in all adult and fetal tissues tested, as well as in many tumor types including ovarian, breast, cervical, melanoma and leukemia. The coding region of MAGE-F1 is contained within a single exon and includes a microsatellite repeat. Several MAGE genes are ubiquitously expressed suggesting a role for MAGE encoded proteins in normal cell physiology.
Application Dilution
WB 1:300-5000
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is MAGEF1 Polyclonal Antibody, PE Conjugated (bs-6231R-PE) validated for, and at what dilution?

bs-6231R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-6231R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise MAGEF1 Polyclonal Antibody, PE Conjugated (bs-6231R-PE)?

KLH conjugated synthetic peptide derived from human MAGEF1

What are the excitation and emission wavelengths of the PE conjugate (bs-6231R-PE)?

PE on bs-6231R-PE: excitation / emission 496,564nm/578nm.

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