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ARK5 Polyclonal Antibody, APC-Cy5.5 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-6251R-APC-Cy5.5-100uL
Product Name ARK5 Polyclonal Antibody, APC-Cy5.5 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Dog, Cow, Pig, Horse, Chicken
Specifications
Conjugation APC-Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ARK5
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9891
Subcellular location Cytoplasm, Nucleus
Synonyms AMPK related protein kinase 5; NUAK family SNF1 like kinase; NUAK1; Probable serine/threonine protein kinase KIAA0537.
Background ARK5, a novel AMPK family member, is the tumor cell survival factor activated by Akt and acts as an ATM kinase under the conditions of nutrient starvation. It plays a key role in tumor malignancy downstream of Akt. Its overexpression is associated with tumor invasion and metastasis.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is ARK5 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-6251R-APC-CY5-5) validated for, and at what dilution?

BS-6251R-APC-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should BS-6251R-APC-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ARK5 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-6251R-APC-CY5-5)?

KLH conjugated synthetic peptide derived from human ARK5

What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BS-6251R-APC-CY5-5)?

APC-Cy5.5 on BS-6251R-APC-CY5-5: excitation / emission 650nm/694nm.

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