JIP1/MAPK8IP1 Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6255R-BF594-100uL |
| Product Name | JIP1/MAPK8IP1 Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human JIP1/MAPK8IP1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9479 |
| Swiss Prot | Q9UQF2 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane, Extracellular matrix |
| Synonyms | IB1; JIP1; JIP-1; PRKM8IP; C-Jun-amino-terminal kinase-interacting protein 1; JNK-interacting protein 1; Islet-brain 1; IB-1; JNK MAP kinase scaffold protein 1; Mitogen-activated protein kinase 8-interacting protein 1; MAPK8IP1 |
| Background | The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Required for JNK activation in response to excitotoxic stress. Cytoplasmic MAPK8IP1 causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and inhibiting JNK phosphorylation of c-Jun. May also participate in ApoER2-specific reelin signaling. Directly, or indirectly, regulates GLUT2 gene expression and beta-cell function. Appears to have a role in cell signaling in mature and developing nerve terminals. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins (By similarity). Functions as an anti-apoptotic protein and whose level seems to influence the beta-cell death or survival response. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is JIP1/MAPK8IP1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-6255R-BF594) validated for, and at what dilution?
bs-6255R-BF594 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does JIP1/MAPK8IP1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-6255R-BF594) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Sheep, Pig, Horse.
How should bs-6255R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise JIP1/MAPK8IP1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-6255R-BF594)?
KLH conjugated synthetic peptide derived from human JIP1/MAPK8IP1
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-6255R-BF594)?
AbBy Fluor-594 on bs-6255R-BF594: excitation / emission 590nm/617nm.