KIST Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-6260R-BF405-100uL |
| Product Name | KIST Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Pig, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human KIST/UHMK1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 127933 |
| Swiss Prot | Q8TAS1 |
| Subcellular location | Nucleus |
| Synonyms | KIS; KIST; P-CIP2; Serine/threonine-protein kinase Kist; Kinase interacting with stathmin; PAM COOH-terminal interactor protein 2; U2AF homology motif kinase 1; UHMK1 |
| Background | Upon serum stimulation, phosphorylates CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. May be involved in trafficking and/or processing of RNA (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is KIST Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-6260R-BF405) validated for, and at what dilution?
bs-6260R-BF405 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-6260R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise KIST Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-6260R-BF405)?
KLH conjugated synthetic peptide derived from human KIST/UHMK1
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-6260R-BF405)?
AbBy Fluor-405 on bs-6260R-BF405: excitation / emission 401nm/421nm.