NEK3 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6261R-Cy3 |
| Product Name | NEK3 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NEK3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4752 |
| Subcellular location | Cytoplasm |
| Synonyms | HSPK 36; HSPK36; HSPK-36; NEK3; NEK 3; NEK-3; NEK3_HUMAN; Never in mitosis A-related kinase 3; NIMA never in mitosis gene a related kinase 3; NimA related protein kinase 3; NimA-related protein kinase 3; Serine/threonine protein kinase Nek3; Serine/threonine-protein kinase Nek3. |
| Background | This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar2009]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NEK3 Polyclonal Antibody, Cy3 Conjugated (bs-6261R-Cy3) validated for, and at what dilution?
bs-6261R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NEK3 Polyclonal Antibody, Cy3 Conjugated (bs-6261R-Cy3) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Pig, Horse, Rabbit.
How should bs-6261R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NEK3 Polyclonal Antibody, Cy3 Conjugated (bs-6261R-Cy3)?
KLH conjugated synthetic peptide derived from human NEK3
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-6261R-Cy3)?
Cy3 on bs-6261R-Cy3: excitation / emission 512,550nm/570,615nm.