phospho-DDR1 (Tyr513) Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6269R-BF680-100uL |
| Product Name | phospho-DDR1 (Tyr513) Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human MCK10 around the phosphorylation site of Tyr513 |
| Modification Site | Tyr513 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 780 |
| Swiss Prot | Q08345 |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | MCK10 phospho Y513; p-MCK10 phospho Y513; CAK; CD167a; DDR1CD 167; CD167; Cell adhesion kinase; DDR 1; DDR; DDR1; Discoidin domain receptor; Discoidin receptor tyrosine kinase isoform a; EDDR 1; Epithelial discoidin domain receptor 1; Epithelial specic receptor kinase; Mammarian carcinoma kinase 10; Neuroepithelial tyrosine kinase; Neurotrophic tyrosine kinase receptor type 4; NTRK 4; PTK 3; PTK 3A protein tyrosine kinase 3A; Receptor tyrosine kinase NEP; RTK 6; TRK E; Tyrosine kinase receptor E; DDR1_HUMAN. |
| Background | Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen. This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain. Its autophosphorylation is achieved by all collagens so far tested (type I to type VI). In situ studies and Northern-blot analysis showed that expression of this encoded protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, this protein is significantly over-expressed in several human tumors from breast, ovarian, esophageal, and pediatric brain. This gene is located on chromosome 6p21.3 in proximity to several HLA class I genes. Alternative splicing of this gene results in multiple transcript variants. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-DDR1 (Tyr513) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-6269R-BF680) validated for, and at what dilution?
bs-6269R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-DDR1 (Tyr513) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-6269R-BF680) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Pig, Horse.
How should bs-6269R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-DDR1 (Tyr513) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-6269R-BF680)?
KLH conjugated synthetic phosphopeptide derived from human MCK10 around the phosphorylation site of Tyr513
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-6269R-BF680)?
AbBy Fluor-680 on bs-6269R-BF680: excitation / emission 679nm/702nm.