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phospho-Ret (Tyr1062) Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Cow
  • Rabbit
Overview
Catalog # bs-6271R-FITC-100uL
Product Name phospho-Ret (Tyr1062) Polyclonal Antibody, FITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Dog, Cow, Rabbit
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human Ret around the phosphorylation site of Tyr1062
Modification Site Tyr1062
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5979
Subcellular location Secreted, Cell membrane
Synonyms Ret phospho Y1062; p-Retphospho Y1062; C ret; Cadherin family member 12; CDHF 12; CDHF12; ELKS Fusion gene; HSCR 1; HSCR1; Hydroxyaryl protein kinase; MEN2A; MEN2B; MTC 1; MTC1; Multiple endocrine neoplasia and medullary thyroid carcinoma 1; Oncogene RET; Proto oncogene tyrosine protein kinase receptor ret; Proto-oncogene c-Ret; Proto-oncogene tyrosine-protein kinase receptor ret; PTC; RET; RET ELE1; Ret Proto oncogene; RET transforming sequence; RET_HUMAN; RET51.
Background Probable receptor with tyrosine-protein kinase activity; important for development.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is phospho-Ret (Tyr1062) Polyclonal Antibody, FITC Conjugated (bs-6271R-FITC) validated for, and at what dilution?

bs-6271R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-6271R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise phospho-Ret (Tyr1062) Polyclonal Antibody, FITC Conjugated (bs-6271R-FITC)?

KLH conjugated synthetic phosphopeptide derived from human Ret around the phosphorylation site of Tyr1062

What are the excitation and emission wavelengths of the FITC conjugate (bs-6271R-FITC)?

FITC on bs-6271R-FITC: excitation / emission 494nm/518nm.