Hepsin/TMPRSS1 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6281R-Cy3 |
| Product Name | Hepsin/TMPRSS1 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Sheep, Pig, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Hepsin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3249 |
| Subcellular location | Cell membrane |
| Synonyms | HEPS; HEPS_HUMAN; Hepsin; HPN; Serine protease hepsin; Serine protease hepsin catalytic chain; Serine protease hepsin non catalytic chain; TMPRSS 1; TMPRSS1; Transmembrane protease serine 1. |
| Background | This gene encodes a type II transmembrane serine protease. The encoded protein has an extracellular region that consists of two domains, a catalytic serine protease domain and a non-catalytic scavenger receptor cysteine-rich domain. This protein may be involved in diverse cellular functions including blood coagulation, maintenance of cell morphology and the growth and progression of certain cancers, particularly prostate cancer. Alternative splicing results in multiple transcript variants. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Hepsin/TMPRSS1 Polyclonal Antibody, Cy3 Conjugated (bs-6281R-Cy3) validated for, and at what dilution?
bs-6281R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Hepsin/TMPRSS1 Polyclonal Antibody, Cy3 Conjugated (bs-6281R-Cy3) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Sheep, Pig, Rabbit.
How should bs-6281R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Hepsin/TMPRSS1 Polyclonal Antibody, Cy3 Conjugated (bs-6281R-Cy3)?
KLH conjugated synthetic peptide derived from human Hepsin
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-6281R-Cy3)?
Cy3 on bs-6281R-Cy3: excitation / emission 512,550nm/570,615nm.