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Hepsin/TMPRSS1 Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Cow
  • Sheep
  • Pig
  • Rabbit
Overview
Catalog # bs-6281R-FITC
Product Name Hepsin/TMPRSS1 Polyclonal Antibody, FITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Cow, Sheep, Pig, Rabbit
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Hepsin
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3249
Subcellular location Cell membrane
Synonyms HEPS; HEPS_HUMAN; Hepsin; HPN; Serine protease hepsin; Serine protease hepsin catalytic chain; Serine protease hepsin non catalytic chain; TMPRSS 1; TMPRSS1; Transmembrane protease serine 1.
Background This gene encodes a type II transmembrane serine protease. The encoded protein has an extracellular region that consists of two domains, a catalytic serine protease domain and a non-catalytic scavenger receptor cysteine-rich domain. This protein may be involved in diverse cellular functions including blood coagulation, maintenance of cell morphology and the growth and progression of certain cancers, particularly prostate cancer. Alternative splicing results in multiple transcript variants.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Hepsin/TMPRSS1 Polyclonal Antibody, FITC Conjugated (bs-6281R-FITC) validated for, and at what dilution?

bs-6281R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Hepsin/TMPRSS1 Polyclonal Antibody, FITC Conjugated (bs-6281R-FITC) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Sheep, Pig, Rabbit.

How should bs-6281R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Hepsin/TMPRSS1 Polyclonal Antibody, FITC Conjugated (bs-6281R-FITC)?

KLH conjugated synthetic peptide derived from human Hepsin

What are the excitation and emission wavelengths of the FITC conjugate (bs-6281R-FITC)?

FITC on bs-6281R-FITC: excitation / emission 494nm/518nm.