Caveolin-2 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-6310R-FITC-100uL |
| Product Name | Caveolin-2 Polyclonal Antibody, FITC Conjugated |
| Applications |
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Caveolin-2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 858 |
| Synonyms | CAV; CAV2; CAV2_HUMAN; Caveolae protein 20 kD; Caveolin 2; Caveolin2; Caveolin 2 isoform a and b; Caveolin 2 isoform c; Caveolin-2; MGC12294. |
| Background | May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.Tissue specificity: Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.Cellular localization: Nucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments. |
| Application Dilution | |
| IF | =1:100-500 |
Frequently asked questions
Which applications is Caveolin-2 Polyclonal Antibody, FITC Conjugated (bs-6310R-FITC) validated for, and at what dilution?
bs-6310R-FITC is validated for IF (IF=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Caveolin-2 Polyclonal Antibody, FITC Conjugated (bs-6310R-FITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-6310R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Caveolin-2 Polyclonal Antibody, FITC Conjugated (bs-6310R-FITC)?
KLH conjugated synthetic peptide derived from human Caveolin-2
What are the excitation and emission wavelengths of the FITC conjugate (bs-6310R-FITC)?
FITC on bs-6310R-FITC: excitation / emission 494nm/518nm.