ALOX15B Polyclonal Antibody, APC-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6335R-APC-Cy5.5-100uL |
| Product Name | ALOX15B Polyclonal Antibody, APC-Cy5.5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | APC-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ALOX15B/15 Lipoxygenase 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 247 |
| Swiss Prot | O15296 |
| Subcellular location | Cytoplasm |
| Synonyms | 15-LOX-2; Arachidonate 15-lipoxygenase B; 15-LOX-B; 15-lipoxygenase 2; Arachidonate 15-lipoxygenase type II; Linoleate 13-lipoxygenase 15-LOb; ALOX15B |
| Background | Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid/(15S)-HPETE. Also acts on linoleic acid to produce 13-hydroxyoctadecadienoic acid/13-HPODE. Has no detectable 8S-lipoxygenase activity but reacts with (8S)-HPETE to produce (8S,15S)-diHPETE. May regulate progression through the cell cycle and cell proliferation. May also regulate cytokine secretion by macrophages and therefore play a role in the immune response. May also regulate macrophage differentiation into proatherogenic foam cells. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ALOX15B Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-6335R-APC-CY5-5) validated for, and at what dilution?
BS-6335R-APC-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ALOX15B Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-6335R-APC-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Pig, Horse, Rabbit.
How should BS-6335R-APC-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ALOX15B Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-6335R-APC-CY5-5)?
KLH conjugated synthetic peptide derived from human ALOX15B/15 Lipoxygenase 2
What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BS-6335R-APC-CY5-5)?
APC-Cy5.5 on BS-6335R-APC-CY5-5: excitation / emission 650nm/694nm.