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DDIT4L Polyclonal Antibody, HRP Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-6376R-HRP
Product Name DDIT4L Polyclonal Antibody, HRP Conjugated
Applications
WB ELISA IHC-P IHC-F
Reactivity Human, Mouse
Predicted Reactivity Rat, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human DDIT4L
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 115265
Swiss Prot Q96D03
Subcellular location Cytoplasm
Synonyms REDD2; Rtp801L; DNA damage-inducible transcript 4-like protein; HIF-1 responsive protein RTP801-like; Protein regulated in development and DNA damage response 2; REDD-2; DDIT4L
Background Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is DDIT4L Polyclonal Antibody, HRP Conjugated (bs-6376R-HRP) validated for, and at what dilution?

bs-6376R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does DDIT4L Polyclonal Antibody, HRP Conjugated (bs-6376R-HRP) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Horse, Rabbit.

How should bs-6376R-HRP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise DDIT4L Polyclonal Antibody, HRP Conjugated (bs-6376R-HRP)?

KLH conjugated synthetic peptide derived from human DDIT4L