Deformed Epidermal Autoregulatory Factor 1 Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6377R-Cy5.5 |
| Product Name | Deformed Epidermal Autoregulatory Factor 1 Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Horse, Chicken |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Deformed Epidermal Autoregulatory Factor 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10522 |
| Subcellular location | Cytoplasm, Nucleus, Secreted |
| Synonyms | DEAF1; Deformed epidermal autoregulatory factor 1 homolog; Nuclear DEA1 related transcriptional regulator; NUDR; SPN; Suppressin; Zinc finger MYND domain containing protein 5; ZMYND5; DEAF1_HUMAN. |
| Background | Suppressin, also known as DEAF1 is a transcription factor required for embryonic development. Suppressin contains one SAND domain and one C-terminal MYND-type zinc finger. It interacts with LMO4 and CLIM-2, suggesting that it plays a role mediating cell fate and embryonic pattern formation. Suppressin is expressed in a variety of tissues and localizes to the nucleus. Several isoforms exist due to alternative splicing and, depending on the isoform, Suppressin is secreted in some cell types. Secreted Suppressin can function to inhibit cell proliferation, arresting cells in the G0 or G1 phase. Mutations in the gene encoding Suppressin may result in a growth advantage leading to the development and progression of neoplasia. This suggest that Supressin is a potential target for cancer therapy. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is Deformed Epidermal Autoregulatory Factor 1 Antibody, Cy5.5 Conjugated (BS-6377R-CY5-5) validated for, and at what dilution?
BS-6377R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Deformed Epidermal Autoregulatory Factor 1 Antibody, Cy5.5 Conjugated (BS-6377R-CY5-5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Horse, Chicken.
How should BS-6377R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Deformed Epidermal Autoregulatory Factor 1 Antibody, Cy5.5 Conjugated (BS-6377R-CY5-5)?
KLH conjugated synthetic peptide derived from human Deformed Epidermal Autoregulatory Factor 1
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-6377R-CY5-5)?
Cy5.5 on BS-6377R-CY5-5: excitation / emission 675nm/694nm.