P15RS Polyclonal Antibody, Cy3 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-6391R-Cy3 |
| Product Name | P15RS Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Pig, Horse |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RPRD1A/P15RS |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Nucleus |
| Synonyms | Cyclin dependent kinase 2B inhibitor related protein; Cyclin dependent kinase inhibitor 2B related protein p15INK4B related protein; Cyclin dependent kinase inhibitor 2B related protein; Cyclin dependent kinase inhibitor related protein; Hypothetical protein FLJ10656; p15INK4B related protein; Putative uncharacterized protein P15RS; Regulation of nuclear pre mRNA domain containing 1A; RPRD1A; RPR1A_HUMAN. |
| Background | P15RS is upregulated in cells overexpressing cyclin-dependent kinase inhibitor p15(INK4b) (CDKN2B; MIM 600431) and may have a role in cell cycle regulation |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is P15RS Polyclonal Antibody, Cy3 Conjugated (bs-6391R-Cy3) validated for, and at what dilution?
bs-6391R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-6391R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise P15RS Polyclonal Antibody, Cy3 Conjugated (bs-6391R-Cy3)?
KLH conjugated synthetic peptide derived from human RPRD1A/P15RS
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-6391R-Cy3)?
Cy3 on bs-6391R-Cy3: excitation / emission 512,550nm/570,615nm.