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phospho-PP2A alpha+beta (Tyr307) Polyclonal Antibody, APC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Dog
  • Cow
  • Pig
  • Chicken
Overview
Catalog # bs-6419R-APC-100uL
Product Name phospho-PP2A alpha+beta (Tyr307) Polyclonal Antibody, APC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Rat
Predicted Reactivity Mouse, Dog, Cow, Pig, Chicken
Specifications
Conjugation APC
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human PPP2CA around the phosphorylation site of Tyr307
Modification Site Tyr307
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5520, 5516
Subcellular location Cytoplasm, Nucleus
Synonyms PP2A alpha phospho Y307; p-PP2A alphaY307; PP2A A; PP2A alpha; PP2A B; PP2A beta; PP2Ac; PP2C alpha; PP2C beta; PP2CA; PP2CB; PPP2CA; PPP2CB; Protein phosphatase 2 formerly 2A catalytic subunit alpha isoform; Protein phosphatase 2 formerly 2A catalytic subunit beta isoform; Protein phosphatase 2; Protein phosphatase 2 catalytic subunit alpha isoform; Protein phosphatase 2 catalytic subunit beta isoform; Protein Phosphatase 2A; Protein phosphatase type 2A catalytic subunit; Replication protein C; RP C; Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform; Serine/threonine protein phosphatase 2A catalytic subunit beta isoform; Serine/threonine protein phosphatase.
Background This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008].
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is phospho-PP2A alpha+beta (Tyr307) Polyclonal Antibody, APC Conjugated (bs-6419R-APC) validated for, and at what dilution?

bs-6419R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does phospho-PP2A alpha+beta (Tyr307) Polyclonal Antibody, APC Conjugated (bs-6419R-APC) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Pig, Chicken.

How should bs-6419R-APC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise phospho-PP2A alpha+beta (Tyr307) Polyclonal Antibody, APC Conjugated (bs-6419R-APC)?

KLH conjugated synthetic phosphopeptide derived from human PPP2CA around the phosphorylation site of Tyr307

What are the excitation and emission wavelengths of the APC conjugate (bs-6419R-APC)?

APC on bs-6419R-APC: excitation / emission 650nm/660nm.

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