phospho-PP2A alpha+beta (Tyr307) Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6419R-BF750-100uL |
| Product Name | phospho-PP2A alpha+beta (Tyr307) Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Pig, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human PPP2CA around the phosphorylation site of Tyr307 |
| Modification Site | Tyr307 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5520, 5516 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | PP2A alpha phospho Y307; p-PP2A alphaY307; PP2A A; PP2A alpha; PP2A B; PP2A beta; PP2Ac; PP2C alpha; PP2C beta; PP2CA; PP2CB; PPP2CA; PPP2CB; Protein phosphatase 2 formerly 2A catalytic subunit alpha isoform; Protein phosphatase 2 formerly 2A catalytic subunit beta isoform; Protein phosphatase 2; Protein phosphatase 2 catalytic subunit alpha isoform; Protein phosphatase 2 catalytic subunit beta isoform; Protein Phosphatase 2A; Protein phosphatase type 2A catalytic subunit; Replication protein C; RP C; Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform; Serine/threonine protein phosphatase 2A catalytic subunit beta isoform; Serine/threonine protein phosphatase. |
| Background | This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-PP2A alpha+beta (Tyr307) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-6419R-BF750) validated for, and at what dilution?
bs-6419R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-PP2A alpha+beta (Tyr307) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-6419R-BF750) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Pig, Chicken.
How should bs-6419R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-PP2A alpha+beta (Tyr307) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-6419R-BF750)?
KLH conjugated synthetic phosphopeptide derived from human PPP2CA around the phosphorylation site of Tyr307
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-6419R-BF750)?
AbBy Fluor-750 on bs-6419R-BF750: excitation / emission 749nm/775nm.