CATSPER4 Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-6422R-BF555-100uL |
| Product Name | CATSPER4 Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CATSPER4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 378807 |
| Swiss Prot | Q7RTX7 |
| Subcellular location | Cell membrane |
| Synonyms | CTSR4_HUMAN; Cation channel sperm-associated protein 4; CatSper4; CatSper-4; cation channel, sperm-associated 4. |
| Background | CatSpers (cation channel, sperm associated proteins) are ion transport proteins located on the surface of sperm cells in the principal piece of the sperm tail. CatSpers are vital to sperm motility, fertilization and cAMP-mediated calcium influx in sperm. There are four CatSper proteins in mammalian sperm, namely CatSper (or CatSper1), CatSper2, CatSper3 and CatSper4. CatSper proteins contain a single, six-transmembrane-spanning segment and exhibit the voltage-dependent Ca2+ channel four-repeat structure. CatSper proteins are believed to assemble into a heterotetrameric complex, forming an alkalinization-activated Ca2+-selective channel. Mutations in any of the genes encoding CatSper family proteins can result in male infertility. CatSper3 plays an important role in the hyperactivated motility of sperm cells, a process that is required in the preparation of sperm for fertilization. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CATSPER4 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-6422R-BF555) validated for, and at what dilution?
bs-6422R-BF555 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-6422R-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CATSPER4 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-6422R-BF555)?
KLH conjugated synthetic peptide derived from human CATSPER4
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-6422R-BF555)?
AbBy Fluor-555 on bs-6422R-BF555: excitation / emission 553nm/568nm.