Lens culinaris agglutinin Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-6439R-Cy3 |
| Product Name | Lens culinaris agglutinin Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Others |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from Lens culinaris Lectin. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Swiss Prot | P02870 |
| Synonyms | Lectin; Lectin precursor |
| Background | Lens culinaris agglutinin is composed of four subunits - two of about 17 kDa and two of 8 kDa. LCA recognizes sequences containing _-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An _-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide markedly enhances affinity. LCA has been found to be one of the most effective agents in preventing skin allograft rejection in model systems. LCA has been used to purify numerous glycoproteins, including immunoglobulins, histocompatibility antigens, and _2-macroglobulin. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Lens culinaris agglutinin Polyclonal Antibody, Cy3 Conjugated (bs-6439R-Cy3) validated for, and at what dilution?
bs-6439R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-6439R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Lens culinaris agglutinin Polyclonal Antibody, Cy3 Conjugated (bs-6439R-Cy3)?
KLH conjugated synthetic peptide derived from Lens culinaris Lectin.
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-6439R-Cy3)?
Cy3 on bs-6439R-Cy3: excitation / emission 512,550nm/570,615nm.