PRLR Polyclonal Antibody, PerCP-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6445r-percp-cy7 |
| Product Name | PRLR Polyclonal Antibody, PerCP-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat, Others |
| Predicted Reactivity | Pig, Rabbit |
| Specifications | |
| Conjugation | PerCP-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Prolactin Receptor |
| Immunogen Range | 251-350/622 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5618 |
| Swiss Prot | P16471 |
| Subcellular location | Cytoplasm |
| Synonyms | HPRL; MFAB; hPRLrI; Prolactin receptor; PRL-R; PRLR |
| Background | This is a receptor for the anterior pituitary hormone prolactin (PRL). Acts as a prosurvival factor for spermatozoa by inhibiting sperm capacitation through suppression of SRC kinase activation and stimulation of AKT. Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PRLR Polyclonal Antibody, PerCP-Cy7 Conjugated (bs-6445R-PerCP-Cy7) validated for, and at what dilution?
bs-6445R-PerCP-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PRLR Polyclonal Antibody, PerCP-Cy7 Conjugated (bs-6445R-PerCP-Cy7) react with?
Tested reactivity: Human, Mouse, Rat, Others. Predicted reactivity (expected from sequence homology but not yet tested): Pig, Rabbit.
How should bs-6445R-PerCP-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PRLR Polyclonal Antibody, PerCP-Cy7 Conjugated (bs-6445R-PerCP-Cy7)?
KLH conjugated synthetic peptide derived from human Prolactin Receptor Immunogen range: 251-350/622.
What are the excitation and emission wavelengths of the PerCP-Cy7 conjugate (bs-6445R-PerCP-Cy7)?
PerCP-Cy7 on bs-6445R-PerCP-Cy7: excitation / emission 482nm/780nm.