phospho-Girdin (Ser1417) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6465R-Cy3-100uL |
| Product Name | phospho-Girdin (Ser1417) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Girdin around the phosphorylation site of Ser1417 |
| Modification Site | Ser1417 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 55704 |
| Swiss Prot | Q3V6T2 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | APE; GIV; GRDN; HkRP1; GIRDIN; KIAA1212; Akt phosphorylation enhancer; Coiled-coil domain-containing protein 88A; G alpha-interacting vesicle-associated protein; Girders of actin filament; Hook-related protein 1; CCDC88A |
| Background | Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB, but does not possess kinase activity itself. Phosphorylation of AKT1/PKB thereby induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation (By similarity). Essential for the integrity of the actin cytoskeleton and for cell migration. Required for formation of actin stress fibers and lamellipodia. May be involved in membrane sorting in the early endosome. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is phospho-Girdin (Ser1417) Polyclonal Antibody, Cy3 Conjugated (bs-6465R-Cy3) validated for, and at what dilution?
bs-6465R-Cy3 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Girdin (Ser1417) Polyclonal Antibody, Cy3 Conjugated (bs-6465R-Cy3) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse.
How should bs-6465R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Girdin (Ser1417) Polyclonal Antibody, Cy3 Conjugated (bs-6465R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human Girdin around the phosphorylation site of Ser1417
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-6465R-Cy3)?
Cy3 on bs-6465R-Cy3: excitation / emission 512,550nm/570,615nm.