kir 6.1 Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6468R-BF405-100uL |
| Product Name | kir 6.1 Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Cow, Pig, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human kir 6.1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3764 |
| Swiss Prot | Q15842 |
| Subcellular location | Cell membrane |
| Synonyms | KIR6.1; uKATP-1; ATP-sensitive inward rectifier potassium channel 8; Inward rectifier K(+) channel Kir6.1; Potassium channel, inwardly rectifying subfamily J member 8; KCNJ8 |
| Background | This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is kir 6.1 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-6468R-BF405) validated for, and at what dilution?
bs-6468R-BF405 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does kir 6.1 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-6468R-BF405) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Pig, Chicken, Rabbit.
How should bs-6468R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise kir 6.1 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-6468R-BF405)?
KLH conjugated synthetic peptide derived from human kir 6.1
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-6468R-BF405)?
AbBy Fluor-405 on bs-6468R-BF405: excitation / emission 401nm/421nm.