ITPR3 Polyclonal Antibody, APC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6471R-APC-100uL |
| Product Name | ITPR3 Polyclonal Antibody, APC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Dog |
| Predicted Reactivity | Human, Rat, Cow, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ITPR3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3710 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | IP3R-III; IP3 receptor isoform 3; 4 antibody 5-trisphosphate receptor; 5-trisphosphate receptor type 3; FLJ36205; Inositol 1; Inositol 1,4,5 trisphosphate receptor type 3; IP3 receptor; IP3R 3; IP3R; IP3R3; ITPR 3; ITPR3; ITPR3_HUMAN; Type 3 inositol 1; Type 3 inositol 1,4,5 trisphosphate receptor; Type 3 InsP3 receptor. |
| Background | Inositol 1,4,5-triphosphate (IP3) functions as a second messenger for a myriad of extracellular stimuli including hormones, growth factors and neurotransmitters. Receptor tyrosine kinases indirectly increase the intracellular levels of IP3 through the activation of phospholipases such as phospholipase C (PLC), which convert phosphatidylinositol-4,5 bisphosphate into IP3 and diacylglycerol (DAG). The inositol 1,4,5-triphosphate receptor, IP3R, acts as an inositol triphosphate (IP3)-gated calcium release channel in a variety of cell types. Three IP3 receptor subtypes have been described and are designated IP3R-I, IP3R-II and IP3R-III. IP3R-I is the predominant IP3R subtype expressed in neuronal tissues and the central nervous system, but is also expressed at high levels in the liver. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ITPR3 Polyclonal Antibody, APC Conjugated (bs-6471R-APC) validated for, and at what dilution?
bs-6471R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ITPR3 Polyclonal Antibody, APC Conjugated (bs-6471R-APC) react with?
Tested reactivity: Mouse, Dog. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Horse, Chicken, Rabbit.
How should bs-6471R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ITPR3 Polyclonal Antibody, APC Conjugated (bs-6471R-APC)?
KLH conjugated synthetic peptide derived from human ITPR3
What are the excitation and emission wavelengths of the APC conjugate (bs-6471R-APC)?
APC on bs-6471R-APC: excitation / emission 650nm/660nm.