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A2BP1 Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Cow
  • Horse
Overview
Catalog # bs-6513R-Cy3
Product Name A2BP1 Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Dog, Cow, Horse
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human A2BP1/Fox1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Cytoplasm, Nucleus
Synonyms A2 BP1; A2BP 1; A2BP; A2BP1; Ataxin 2 binding protein 1; Ataxin-2-binding protein 1; Ataxin2 binding protein 1; FOX 1; Fox-1 homolog A; FOX1; RFOX1_HUMAN; Hexaribonucleotide binding protein 1; Hexaribonucleotide-binding protein 1; HRNBP 1; HRNBP1.
Background RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis.Predominantly expressed in muscle and brain.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is A2BP1 Polyclonal Antibody, Cy3 Conjugated (bs-6513R-Cy3) validated for, and at what dilution?

bs-6513R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-6513R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise A2BP1 Polyclonal Antibody, Cy3 Conjugated (bs-6513R-Cy3)?

KLH conjugated synthetic peptide derived from human A2BP1/Fox1

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-6513R-Cy3)?

Cy3 on bs-6513R-Cy3: excitation / emission 512,550nm/570,615nm.