HSPA6 Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-6540R-BF555-100uL |
| Product Name | HSPA6 Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Sheep |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HSPA6 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3310 |
| Subcellular location | Cytoplasm, Secreted, Extracellular matrix |
| Synonyms | Heat shock 70 kDa protein 6; Heat shock 70 kDa protein B; heat shock 70kD protein 6 HSP70B'; Heat shock 70kDa protein 6; HSP70B; HSP76_HUMAN; HSPA6. |
| Background | In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Sequence similarities Belongs to the heat shock protein 70 family. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HSPA6 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-6540R-BF555) validated for, and at what dilution?
bs-6540R-BF555 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-6540R-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HSPA6 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-6540R-BF555)?
KLH conjugated synthetic peptide derived from human HSPA6
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-6540R-BF555)?
AbBy Fluor-555 on bs-6540R-BF555: excitation / emission 553nm/568nm.