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OLFM4 Polyclonal Antibody, APC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Pig
  • Rabbit
Overview
Catalog # bs-6558R-APC
Product Name OLFM4 Polyclonal Antibody, APC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Pig, Rabbit
Specifications
Conjugation APC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human OLFM4
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 10562
Swiss Prot Q6UX06
Subcellular location Cytoplasm, Secreted, Cell membrane, Extracellular matrix
Synonyms GC1; OLM4; OlfD; GW112; hGC-1; hOLfD; UNQ362; bA209J19.1; Olfactomedin-4; Antiapoptotic protein GW112; G-CSF-stimulated clone 1 protein; OLFM4; UNQ362/PRO698
Background May promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. May play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is OLFM4 Polyclonal Antibody, APC Conjugated (bs-6558R-APC) validated for, and at what dilution?

bs-6558R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does OLFM4 Polyclonal Antibody, APC Conjugated (bs-6558R-APC) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Rabbit.

How should bs-6558R-APC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise OLFM4 Polyclonal Antibody, APC Conjugated (bs-6558R-APC)?

KLH conjugated synthetic peptide derived from human OLFM4

What are the excitation and emission wavelengths of the APC conjugate (bs-6558R-APC)?

APC on bs-6558R-APC: excitation / emission 650nm/660nm.