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DUT Polyclonal Antibody, HRP Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-6584R-HRP-100uL
Product Name DUT Polyclonal Antibody, HRP Conjugated
Applications
WB ELISA IHC-P IHC-F
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human DUT
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1854
Swiss Prot P33316
Subcellular location Cytoplasm, Nucleus
Synonyms dUTPase; Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial; dUTP pyrophosphatase; DUT
Background This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is DUT Polyclonal Antibody, HRP Conjugated (bs-6584R-HRP) validated for, and at what dilution?

bs-6584R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does DUT Polyclonal Antibody, HRP Conjugated (bs-6584R-HRP) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit.

How should bs-6584R-HRP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise DUT Polyclonal Antibody, HRP Conjugated (bs-6584R-HRP)?

KLH conjugated synthetic peptide derived from human DUT