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Desmoglein 3 Polyclonal Antibody, AbBy Fluor-750 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Rabbit
Overview
Catalog # bs-6585R-BF750-100uL
Product Name Desmoglein 3 Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Rabbit
Specifications
Conjugation AbBy Fluor-750
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Desmoglein 3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1830
Swiss Prot P32926
Subcellular location Cell membrane
Synonyms PVA; CDHF6; Desmoglein-3; 13 kDa pemphigus vulgaris antigen; Cadherin family member 6; DSG3
Background Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Desmoglein 3 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-6585R-BF750) validated for, and at what dilution?

bs-6585R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Desmoglein 3 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-6585R-BF750) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Rabbit.

How should bs-6585R-BF750 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Desmoglein 3 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-6585R-BF750)?

KLH conjugated synthetic peptide derived from human Desmoglein 3

What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-6585R-BF750)?

AbBy Fluor-750 on bs-6585R-BF750: excitation / emission 749nm/775nm.

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