APEX2 Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-6587R-100uL |
| Product Name | APEX2 Polyclonal Antibody |
| Applications |
WB
ELISA
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human APEX2 |
| Immunogen Range | 301-400/518 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 27301 |
| Swiss Prot | Q9UBZ4 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | APE2; XTH2; ZGRF2; APEXL2; DNA-(apurinic or apyrimidinic site) lyase 2; AP endonuclease XTH2; APEX nuclease 2; APEX nuclease-like 2; Apurinic-apyrimidinic endonuclease 2; AP endonuclease 2; APEX2 |
| Background | Function as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Displays also double-stranded DNA 3'-5' exonuclease, 3'-phosphodiesterase activities. Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially. Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents. In the nucleus functions in the PCNA-dependent BER pathway. Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes. Required for proper cell cycle progression during proliferation of peripheral lymphocytes. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
Frequently asked questions
Which applications is APEX2 Polyclonal Antibody (bs-6587R) validated for, and at what dilution?
bs-6587R is validated for WB (1:300-5000); ELISA (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does APEX2 Polyclonal Antibody (bs-6587R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-6587R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise APEX2 Polyclonal Antibody (bs-6587R)?
KLH conjugated synthetic peptide derived from human APEX2 Immunogen range: 301-400/518.