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Galectin 1 Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Cow
  • Sheep
  • Pig
  • Horse
Overview
Catalog # bs-6594R-FITC-100uL
Product Name Galectin 1 Polyclonal Antibody, FITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Cow, Sheep, Pig, Horse
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Galectin 1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3956
Swiss Prot P09382
Subcellular location Secreted, Extracellular matrix
Synonyms GBP; GAL1; Galectin-1; Gal-1; 14 kDa laminin-binding protein; HLBP14; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; HBL; HPL; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; Putative MAPK-activating protein PM12; S-Lac lectin 1; LGALS1
Background May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Galectin 1 Polyclonal Antibody, FITC Conjugated (bs-6594R-FITC) validated for, and at what dilution?

bs-6594R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Galectin 1 Polyclonal Antibody, FITC Conjugated (bs-6594R-FITC) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Sheep, Pig, Horse.

How should bs-6594R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Galectin 1 Polyclonal Antibody, FITC Conjugated (bs-6594R-FITC)?

KLH conjugated synthetic peptide derived from human Galectin 1

What are the excitation and emission wavelengths of the FITC conjugate (bs-6594R-FITC)?

FITC on bs-6594R-FITC: excitation / emission 494nm/518nm.