HMPH Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HHEX Polyclonal Antibody, Unconjugated (bs-6600R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HHEX Polyclonal Antibody, Unconjugated (bs-6600R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HHEX Polyclonal Antibody, Unconjugated (bs-6600R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HHEX Polyclonal Antibody, Unconjugated (bs-6600R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.

Applications

WB
ELISA
IHC-P

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Pig
Horse
Rabbit

Overview
Catalog #	bs-6600R
Product Name	HMPH Polyclonal Antibody
Applications	WB, ELISA, IHC-P
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human HHEX
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3087
Swiss Prot	Q03014
Subcellular location	Nucleus
Synonyms	Hematopoietically expressed homeobox; Hematopoietically-expressed homeobox protein HHEX; HEX; HHEX; HHEX_HUMAN; HMPH; Homeobox protein HEX; Homeobox protein PRH; HOX11L PEN; PRH; PRHX.
Background	Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400