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Adenosine deaminase Polyclonal Antibody, AbBy Fluor-555 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Cow
  • Pig
  • Horse
Overview
Catalog # bs-6654R-BF555-100uL
Product Name Adenosine deaminase Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Cow, Pig, Horse
Specifications
Conjugation AbBy Fluor-555
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Adenosine deaminase
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 100
Swiss Prot P00813
Subcellular location Cytoplasm, Cell membrane, Extracellular matrix
Synonyms Adenosine deaminase; Adenosine aminohydrolase; ADA; ADA1
Background Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Adenosine deaminase Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-6654R-BF555) validated for, and at what dilution?

bs-6654R-BF555 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Adenosine deaminase Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-6654R-BF555) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Pig, Horse.

How should bs-6654R-BF555 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Adenosine deaminase Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-6654R-BF555)?

KLH conjugated synthetic peptide derived from human Adenosine deaminase

What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-6654R-BF555)?

AbBy Fluor-555 on bs-6654R-BF555: excitation / emission 553nm/568nm.

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