AQP0 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-6661R-BF647-100uL |
| Product Name | AQP0 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Guinea Pig |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human AQP0 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4284 |
| Swiss Prot | P30301 |
| Subcellular location | Cell membrane |
| Synonyms | AQP0; LIM1; MP26; MIP26; CTRCT15; Lens fiber major intrinsic protein; Aquaporin-0; MIP |
| Background | Water channel (PubMed:24120416). Channel activity is down-regulated by CALM when cytoplasmic Ca(2+) levels are increased. May be responsible for regulating the osmolarity of the lens. Interactions between homotetramers from adjoining membranes may stabilize cell junctions in the eye lens core (By similarity). Plays a role in cell-to-cell adhesion and facilitates gap junction coupling (PubMed:24120416). |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is AQP0 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-6661R-BF647) validated for, and at what dilution?
bs-6661R-BF647 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-6661R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise AQP0 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-6661R-BF647)?
KLH conjugated synthetic peptide derived from human AQP0
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-6661R-BF647)?
AbBy Fluor-647 on bs-6661R-BF647: excitation / emission 650nm/665nm.