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HRH1 Polyclonal Antibody, PE-Cy5 Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Pig
Overview
Catalog # bs-6663R-PE-Cy5-100uL
Product Name HRH1 Polyclonal Antibody, PE-Cy5 Conjugated
Applications
WB FCM
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Pig
Specifications
Conjugation PE-Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human HRH1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3269
Swiss Prot P35367
Subcellular location Cell membrane
Synonyms H1-R; hisH1; Histamine H1 receptor; H1R; HH1R; HRH1
Background In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system.
Application Dilution
WB 1:300-5000
FCM 1:20-100

Frequently asked questions

Which applications is HRH1 Polyclonal Antibody, PE-Cy5 Conjugated (bs-6663R-PE-Cy5) validated for, and at what dilution?

bs-6663R-PE-Cy5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HRH1 Polyclonal Antibody, PE-Cy5 Conjugated (bs-6663R-PE-Cy5) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig.

How should bs-6663R-PE-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HRH1 Polyclonal Antibody, PE-Cy5 Conjugated (bs-6663R-PE-Cy5)?

KLH conjugated synthetic peptide derived from human HRH1

What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-6663R-PE-Cy5)?

PE-Cy5 on bs-6663R-PE-Cy5: excitation / emission 488nm/670nm.

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