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HRH2 Polyclonal Antibody

Lane 1: Mouse Small intestine cell lysates probed with HRH2 Polyclonal Antibody, Unconjugated (bs-6664R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Mouse Small intestine cell lysates probed with HRH2 Polyclonal Antibody, Unconjugated (bs-6664R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HRH2 Polyclonal Antibody, Unconjugated (bs-6664R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HRH2 Polyclonal Antibody, Unconjugated (bs-6664R) at 1:400 overnight at 4\u00b0C, DAB staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Dog
  • Rabbit
Overview
Catalog # bs-6664R
Product Name HRH2 Polyclonal Antibody
Applications
WB IHC-P IHC-F IF
Reactivity Human, Mouse
Predicted Reactivity Rat, Dog, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human HRH2
Immunogen Range 161-259/359
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 3274
Swiss Prot P25021
Subcellular location Cell membrane
Synonyms H2R; Histamine H2 receptor; HH2R; Gastric receptor I; HRH2
Background The H2 subclass of histamine receptors mediates gastric acid secretion. Also appears to regulate gastrointestinal motility and intestinal secretion. Possible role in regulating cell growth and differentiation. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and, through a separate G protein-dependent mechanism, the phosphoinositide/protein kinase (PKC) signaling pathway (By similarity).
Application Dilution
WB 1:300-5000
IHC-P ELISA1:500-1000
IHC-F IHC-P1:200-400
IF

Frequently asked questions

Which applications is HRH2 Polyclonal Antibody (bs-6664R) validated for, and at what dilution?

bs-6664R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HRH2 Polyclonal Antibody (bs-6664R) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Rabbit.

How should bs-6664R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HRH2 Polyclonal Antibody (bs-6664R)?

KLH conjugated synthetic peptide derived from human HRH2 Immunogen range: 161-259/359.

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