GPR48 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-6676r-apc-cy7 |
| Product Name | GPR48 Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat, Bovine, Dog, Pig, Chicken |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human LGR4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 55366 |
| Subcellular location | Extracellular |
| Synonyms | G protein coupled receptor 48; G-protein coupled receptor 48; GPR48; GPCR GPR48; Leucine-rich repeat-containing G protein-coupled receptor 4; Leucine-rich repeat-containing G-protein coupled receptor 4; LGR4; LGR4_HUMAN. |
| Background | G protein-coupled receptors (GPCRs), also designated seven transmembrane (7TM) receptors or heptahelical receptors, interact with G proteins (heterotrimeric GTPases) to synthesize intracellular second messengers, such as diacylglycerol, cyclic AMP, inositol phosphates and calcium ions. Their diverse biological functions range from vision and olfaction to neuronal and endocrine signaling and are involved in many pathological conditions. LGR4 (leucine-rich repeat-containing G protein-coupled receptor 4), also known as GPR48, is a 951 amino acid multi-pass membrane protein that contains 15 LRR (leucine-rich repeats) and belongs to the GPCR family. Expressed in multiple tissues, including testis, ovary, placenta, stomach, heart, kidney, pancreas and spleen, LGR4 functions as an orphan receptor that may be involved in physiologic activities throughout the cell. LGR4 is overexpressed in various cancer types and is thought to enhance carcinoma invasiveness and metastasis, suggesting an important role in tumor progression. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is GPR48 Polyclonal Antibody, APC-Cy7 Conjugated (bs-6676R-APC-Cy7) validated for, and at what dilution?
bs-6676R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GPR48 Polyclonal Antibody, APC-Cy7 Conjugated (bs-6676R-APC-Cy7) react with?
Tested reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken.
How should bs-6676R-APC-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GPR48 Polyclonal Antibody, APC-Cy7 Conjugated (bs-6676R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human LGR4
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-6676R-APC-Cy7)?
APC-Cy7 on bs-6676R-APC-Cy7: excitation / emission 650nm/780nm.