GPR48 Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-6676r-100uL |
| Product Name | GPR48 Polyclonal Antibody |
| Applications |
WB
IHC-P
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat, Bovine, Dog, Pig, Chicken |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human LGR4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 55366 |
| Subcellular location | Extracellular |
| Synonyms | G protein coupled receptor 48; G-protein coupled receptor 48; GPR48; GPCR GPR48; Leucine-rich repeat-containing G protein-coupled receptor 4; Leucine-rich repeat-containing G-protein coupled receptor 4; LGR4; LGR4_HUMAN. |
| Background | G protein-coupled receptors (GPCRs), also designated seven transmembrane (7TM) receptors or heptahelical receptors, interact with G proteins (heterotrimeric GTPases) to synthesize intracellular second messengers, such as diacylglycerol, cyclic AMP, inositol phosphates and calcium ions. Their diverse biological functions range from vision and olfaction to neuronal and endocrine signaling and are involved in many pathological conditions. LGR4 (leucine-rich repeat-containing G protein-coupled receptor 4), also known as GPR48, is a 951 amino acid multi-pass membrane protein that contains 15 LRR (leucine-rich repeats) and belongs to the GPCR family. Expressed in multiple tissues, including testis, ovary, placenta, stomach, heart, kidney, pancreas and spleen, LGR4 functions as an orphan receptor that may be involved in physiologic activities throughout the cell. LGR4 is overexpressed in various cancer types and is thought to enhance carcinoma invasiveness and metastasis, suggesting an important role in tumor progression. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is GPR48 Polyclonal Antibody (bs-6676R) validated for, and at what dilution?
bs-6676R is validated for WB (1:300-5000); IHC-P (1:200-400); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GPR48 Polyclonal Antibody (bs-6676R) react with?
Tested reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken.
How should bs-6676R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GPR48 Polyclonal Antibody (bs-6676R)?
KLH conjugated synthetic peptide derived from human LGR4