VMAT1 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6677R-Cy3 |
| Product Name | VMAT1 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human VMAT1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6570 |
| Swiss Prot | P54219 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Adrenal chromaffin granule amine transporter; CGAT; CGAT; Chromaffin granule amine transporter; SLC18A1; Solute carrier family 18 vesicular monoamine, member 1; Solute carrier family 18 member 1; VAT 1; VAT1; Vesicular amine transporter 1; VMAT 1; VMAT1; VMAT1_HUMAN. |
| Background | The vesicular monoamine transporter acts to accumulate cytosolic monoamines into vesicles, using the proton gradient maintained across the vesicular membrane. Its proper function is essential to the correct activity of the monoaminergic systems that have been implicated in several human neuropsychiatric disorders. The transporter is a site of action of important drugs, including reserpine and tetrabenazine (Peter et al., 1993 [PubMed 7905859]). See also SLC18A2 (MIM 193001).[supplied by OMIM, Mar 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is VMAT1 Polyclonal Antibody, Cy3 Conjugated (bs-6677R-Cy3) validated for, and at what dilution?
bs-6677R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does VMAT1 Polyclonal Antibody, Cy3 Conjugated (bs-6677R-Cy3) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig, Horse, Chicken.
How should bs-6677R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise VMAT1 Polyclonal Antibody, Cy3 Conjugated (bs-6677R-Cy3)?
KLH conjugated synthetic peptide derived from human VMAT1
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-6677R-Cy3)?
Cy3 on bs-6677R-Cy3: excitation / emission 512,550nm/570,615nm.