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Transglutaminase-1 Polyclonal Antibody, AbBy Fluor-647 Conjugated

Applications

  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Dog
  • Pig
Overview
Catalog # bs-6690R-BF647
Product Name Transglutaminase-1 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Rat
Predicted Reactivity Mouse, Dog, Pig
Specifications
Conjugation AbBy Fluor-647
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Transglutaminase
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7051
Swiss Prot P22735
Subcellular location Cell membrane
Synonyms LI; KTG; LI1; TGK; ICR2; ARCI1; TGASE; Protein-glutamine gamma-glutamyltransferase K; Epidermal TGase; Transglutaminase K; TG(K); TGase K; Transglutaminase-1; TGase-1; TGM1
Background Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Responsible for cross-linking epidermal proteins during formation of the stratum corneum.
Application Dilution
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Transglutaminase-1 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-6690R-BF647) validated for, and at what dilution?

bs-6690R-BF647 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Transglutaminase-1 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-6690R-BF647) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Pig.

How should bs-6690R-BF647 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Transglutaminase-1 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-6690R-BF647)?

KLH conjugated synthetic peptide derived from human Transglutaminase

What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-6690R-BF647)?

AbBy Fluor-647 on bs-6690R-BF647: excitation / emission 650nm/665nm.