Sertad1 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6691R-Cy5.5-100uL |
| Product Name | Sertad1 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Pig, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Sertad1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 29950 |
| Swiss Prot | Q9UHV2 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | SEI1; TRIP-Br1; SERTA domain-containing protein 1; CDK4-binding protein p34SEI1; SEI-1; Transcriptional regulator interacting with the PHD-bromodomain 1; SERTAD1 |
| Background | Acts at E2F-responsive promoters as coregulator to integrate signals provided by PHD- and/or bromodomain-containing transcription factors. Stimulates E2F1/TFDP1 transcriptional activity. Renders the activity of cyclin D1/CDK4 resistant to the inhibitory effects of CDKN2A/p16INK4A. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Sertad1 Polyclonal Antibody, Cy5.5 Conjugated (BS-6691R-CY5-5) validated for, and at what dilution?
BS-6691R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Sertad1 Polyclonal Antibody, Cy5.5 Conjugated (BS-6691R-CY5-5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Rabbit.
How should BS-6691R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Sertad1 Polyclonal Antibody, Cy5.5 Conjugated (BS-6691R-CY5-5)?
KLH conjugated synthetic peptide derived from human Sertad1
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-6691R-CY5-5)?
Cy5.5 on BS-6691R-CY5-5: excitation / emission 675nm/694nm.