AP2 gamma Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6694R-Cy3-100uL |
| Product Name | AP2 gamma Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TFAP2C |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7022 |
| Swiss Prot | Q92754 |
| Subcellular location | Nucleus |
| Synonyms | ERF1; TFAP2G; hAP-2g; AP2-GAMMA; Transcription factor AP-2 gamma; Activating enhancer-binding protein 2 gamma; Transcription factor ERF-1; TFAP2C |
| Background | Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is AP2 gamma Polyclonal Antibody, Cy3 Conjugated (bs-6694R-Cy3) validated for, and at what dilution?
bs-6694R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does AP2 gamma Polyclonal Antibody, Cy3 Conjugated (bs-6694R-Cy3) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Rabbit.
How should bs-6694R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise AP2 gamma Polyclonal Antibody, Cy3 Conjugated (bs-6694R-Cy3)?
KLH conjugated synthetic peptide derived from human TFAP2C
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-6694R-Cy3)?
Cy3 on bs-6694R-Cy3: excitation / emission 512,550nm/570,615nm.