NEDD5 / septin 2 Polyclonal Antibody, RBITC Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-6704R-RBITC-100uL |
| Product Name | NEDD5 / septin 2 Polyclonal Antibody, RBITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Septin 2/NEDD5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4735 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | DF6; hNEDD5; KIAA0158; Neural precursor cell expressed developmentally down regulated protein 5; Pnutl3; Protein NEDD5; SEPT2; SEPT2_HUMAN. |
| Background | The septins are a family of cytoskeletal GTPases that play an essential role in cytokinesis in mammalian cells. Septin 2 (Nedd5) is a mammalian septin known to associate with actin based structures such as the contractile ring and stress fibers. It is involved in cytokinesis and may assemble into a multicomponent structure. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NEDD5 / septin 2 Polyclonal Antibody, RBITC Conjugated (bs-6704R-RBITC) validated for, and at what dilution?
bs-6704R-RBITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-6704R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NEDD5 / septin 2 Polyclonal Antibody, RBITC Conjugated (bs-6704R-RBITC)?
KLH conjugated synthetic peptide derived from human Septin 2/NEDD5
What are the excitation and emission wavelengths of the RBITC conjugate (bs-6704R-RBITC)?
RBITC on bs-6704R-RBITC: excitation / emission 540nm/625nm.