Netrin G1 ligand Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat brain tissue labeled with Rabbit Anti-Netrin G1 Ligand Polyclonal Antibody, Unconjugated (bs-6710R) at 1:200 followed by Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37\u00b0C.$

Formalin-fixed and paraffin embedded rat brain tissue labeled with Rabbit Anti-Netrin G1 Ligand Polyclonal Antibody, Unconjugated (bs-6710R) at 1:200 followed by Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37\u00b0C.

Hela lysates probed with Netrin G1 ligand Polyclonal Antibody, Unconjugated (bs-6710R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Horse
Rabbit

Overview
Catalog #	bs-6710R
Product Name	Netrin G1 ligand Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Netrin G1 ligand
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	22854
Subcellular location	Cell membrane
Synonyms	Leucine rich repeat containing 4C; Leucine-rich repeat-containing protein 4C; LRC4C_HUMAN; Lrrc4C; Netrin-G1 ligand; NGL 1; NGL-1; NGL1.
Background	NGL-1 is a single pass type I membrane protein that acts as a cell adhesion molecule. It contains nine leucine-rich repeats (LRR) and one Ig-like C2-type domain. NGL-1 is predominantly expressed in the striatum and the cerebral cortex of both the embryonic and adult brain. NGL-1 specifically interacts with Netrin G1 (a molecule involved in axon guidance in the developing central nervous system) via its LRR region. NGL-1 plays a role in the regulation of neurite outgrowth of developing thalamic neurons. Soluble NGL-1 inhibits thalamic axon outgrowth while NGL-1 that is bound to the surface of developing thalamocortical axons stimulates growth. NGL-1 also interacts with Whirlin possibly stablizing interstereociliar links.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200