phospho-CAMK4 (Thr196 + Thr200) Polyclonal Antibody, Cy5.5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-6728R-Cy5.5-100uL |
| Product Name | phospho-CAMK4 (Thr196 + Thr200) Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | Phosphorylation site corresponds to Thr200 and Thr204 in human. |
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from mouse CAMKIV around the phosphorylation site of Thr196 + Thr200. |
| Modification Site | Thr196 + Thr200 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Swiss Prot | P08414 |
| Subcellular location | Nucleus |
| Synonyms | CAMKIVphospho T196 + T200; Brain Ca2+ calmodulin dependent protein kinase type 4; Brain Ca2+ calmodulin dependent protein kinase type IV; Calcium / calmodulin dependent protein kinase type 4 catalytic chain; Calcium / calmodulin dependent protein kinase type IV catalytic chain; Calcium/calmodulin dependent protein kinase IV; Calcium/calmodulin dependent protein kinase type IV; CAM kinase 4; CAM kinase GR; CAM kinase IV; CaMK 4; CAMK GR; CaMK IV; CaMK4; CaMKGR; MGC36771. |
| Background | Ca2+/calmodulin dependent protein kinase type IV (CAMKIV) belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-CAMK4 (Thr196 + Thr200) Polyclonal Antibody, Cy5.5 Conjugated (BS-6728R-CY5-5) validated for, and at what dilution?
BS-6728R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should BS-6728R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-CAMK4 (Thr196 + Thr200) Polyclonal Antibody, Cy5.5 Conjugated (BS-6728R-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from mouse CAMKIV around the phosphorylation site of Thr196 + Thr200.
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-6728R-CY5-5)?
Cy5.5 on BS-6728R-CY5-5: excitation / emission 675nm/694nm.