Help

Upstream Binding Protein 1 Antibody, APC-Cy5.5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-6730R-APC-Cy5.5-100uL
Product Name Upstream Binding Protein 1 Antibody, APC-Cy5.5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Pig, Horse, Chicken
Specifications
Conjugation APC-Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Upstream Binding Protein 1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7342
Subcellular location Nucleus
Synonyms LBP 1a; Cp2b; DKFZp686L1745; LBP 1a; LBP 1B; LBP1; LBP1A; LBP1B; NF2d9; Upstream binding protein 1; Transcription factor LBP-1; UBIP1_HUMAN.
Background Functions as a transcriptional activator in a promoter context-dependent manner. Modulates the placental expression of CYP11A1. Involved in regulation of the alpha-globin gene in erythroid cells. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with TFCP2 (By similarity). Involved in regulation of the alpha-globin gene in erythroid cells. Binds strongly to sequences around the HIV-1 initiation site and weakly over the TATA-box. Represses HIV-1 transcription by inhibiting the binding of TFIID to the TATA-box.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Upstream Binding Protein 1 Antibody, APC-Cy5.5 Conjugated (BS-6730R-APC-CY5-5) validated for, and at what dilution?

BS-6730R-APC-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Upstream Binding Protein 1 Antibody, APC-Cy5.5 Conjugated (BS-6730R-APC-CY5-5) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse, Chicken.

How should BS-6730R-APC-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Upstream Binding Protein 1 Antibody, APC-Cy5.5 Conjugated (BS-6730R-APC-CY5-5)?

KLH conjugated synthetic peptide derived from human Upstream Binding Protein 1

What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BS-6730R-APC-CY5-5)?

APC-Cy5.5 on BS-6730R-APC-CY5-5: excitation / emission 650nm/694nm.