PDCD6IP Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-6767R-BF488-100uL |
| Product Name | PDCD6IP Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PDC6I |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10015 |
| Subcellular location | Cytoplasm |
| Synonyms | AIP1; ALG 2 interacting protein 1; ALG-2-interacting protein 1; ALG2 interacting protein X; Alix; Apoptosis linked gene 2 interacting protein X; Dopamine receptor interacting protein 4; DRIP4; HP95; KIAA1375; MGC17003; PDC6I_HUMAN; PDCD6 interacting protein; PDCD6-interacting protein; PDCD6IP; Programmed cell death 6 interacting protein; Programmed cell death 6-interacting protein. |
| Background | Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function implies the interaction with CHMP4B. May play a role in the regulation of both apoptosis and cell proliferation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PDCD6IP Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-6767R-BF488) validated for, and at what dilution?
bs-6767R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PDCD6IP Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-6767R-BF488) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-6767R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PDCD6IP Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-6767R-BF488)?
KLH conjugated synthetic peptide derived from human PDC6I
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-6767R-BF488)?
AbBy Fluor-488 on bs-6767R-BF488: excitation / emission 499nm/519nm.