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PHAPI2 Polyclonal Antibody, Cy5 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Dog
  • Sheep
  • Zebrafish

Predicted Reactivity

  • Mouse
  • Rat
  • Cow
  • Fruit Fly
Overview
Catalog # bs-6769r-cy5-100uL
Product Name PHAPI2 Polyclonal Antibody, Cy5 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Dog, Sheep, Zebrafish
Predicted Reactivity Mouse, Rat, Cow, Fruit Fly
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PHAPI2/APRIL
Immunogen Range 51-150/251
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 10541
Subcellular location Cytoplasm, Nucleus
Synonyms Acidic leucine rich nuclear phosphoprotein 32 family member B; Acidic leucine rich nuclear phosphoprotein 32 family member B; Acidic leucine-rich nuclear phosphoprotein 32 family member B; Acidic nuclear phosphoprotein 32 family member B; Acidic protein rich in leucines; AN32B_HUMAN; ANP 32B; ANP32B; APRIL; PAL 31; PAL31; PHAPI 2; PHAPI2; PHAPI2 protein; PHAPI2a; proliferation related acidic leucine rich protein; Putative HLA-DR-associated protein I-2; Silver stainable protein SSP 29; Silver stainable protein SSP29; Silver-stainable protein SSP29; Ssp 29; Ssp29.
Background Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity (By similarity). Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is PHAPI2 Polyclonal Antibody, Cy5 Conjugated (bs-6769R-Cy5) validated for, and at what dilution?

bs-6769R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PHAPI2 Polyclonal Antibody, Cy5 Conjugated (bs-6769R-Cy5) react with?

Tested reactivity: Human, Dog, Sheep, Zebrafish. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Fruit Fly.

How should bs-6769R-Cy5 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PHAPI2 Polyclonal Antibody, Cy5 Conjugated (bs-6769R-Cy5)?

KLH conjugated synthetic peptide derived from human PHAPI2/APRIL Immunogen range: 51-150/251.

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-6769R-Cy5)?

Cy5 on bs-6769R-Cy5: excitation / emission 625,650nm/670nm.