phospho-MAP3K9+MAP3K10 (Thr312 + Thr266) Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6779R-APC-Cy7 |
| Product Name | phospho-MAP3K9+MAP3K10 (Thr312 + Thr266) Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human MAP3K9 around the phosphorylation site of Thr312 |
| Modification Site | Thr312 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4293 |
| Swiss Prot | P80192 |
| Subcellular location | Cytoplasm |
| Synonyms | MLK1; MEKK9; PRKE1; Mitogen-activated protein kinase kinase kinase 9; Mixed lineage kinase 1; MAP3K9 |
| Background | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Plays also a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. |
| Application Dilution | |
| WB | |
| IF(IHC-P) | |
| IF(IHC-F) | |
| IF(ICC) | |
Frequently asked questions
Which applications is phospho-MAP3K9+MAP3K10 (Thr312 + Thr266) Polyclonal Antibody, APC-Cy7 Conjugated (bs-6779R-APC-Cy7) validated for, and at what dilution?
bs-6779R-APC-Cy7 is validated for WB, IF(IHC-P), IF(IHC-F), IF(ICC).
Which species does phospho-MAP3K9+MAP3K10 (Thr312 + Thr266) Polyclonal Antibody, APC-Cy7 Conjugated (bs-6779R-APC-Cy7) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit.
How should bs-6779R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-MAP3K9+MAP3K10 (Thr312 + Thr266) Polyclonal Antibody, APC-Cy7 Conjugated (bs-6779R-APC-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human MAP3K9 around the phosphorylation site of Thr312