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Calpain 12 Polyclonal Antibody, Biotin Conjugated

Applications

  • ELISA
  • IHC-P
  • IHC-F

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Cow
  • Pig
Overview
Catalog # bs-6798R-Biotin-100uL
Product Name Calpain 12 Polyclonal Antibody, Biotin Conjugated
Applications
ELISA IHC-P IHC-F
Predicted Reactivity Human, Mouse, Rat, Dog, Cow, Pig
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Calpain 12
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C for 12 months.
Target
Gene ID 147968
Subcellular location Cytoplasm
Synonyms Calpain12; Calpain-12; CAPN 12; CAPN12; EC 3.4.22.; MGC20576; CAN12_HUMAN.
Background Calpains are a family of cytosolic calcium activated cysteine proteases involved in a variety of cellular processes including apoptosis, cell division, modulation of integrin and cytoskeletal interactions, and synaptic plasticity. Calpain 12 was first described in the mouse, most strongly in the skin, and maps to mouse chromosome 7. Isoforms differ in the carboxyterminal ends, ending with aberrant domain III and lacking domain IV. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV, making Calpain 12 most similar to calpains 1 and 2.
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is Calpain 12 Polyclonal Antibody, Biotin Conjugated (bs-6798R-Biotin) validated for, and at what dilution?

bs-6798R-Biotin is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-6798R-Biotin be stored?

Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Calpain 12 Polyclonal Antibody, Biotin Conjugated (bs-6798R-Biotin)?

KLH conjugated synthetic peptide derived from human Calpain 12

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